Purpose: The purpose of this science laboratory is to test for enzyme activity, enumerate at enzyme specificity, and how temperature affects enzyme activity.
Time need to perform this lab: some 3 hours
Preparation
FIRST: Read the lab in its entirety TWICE before you begin. You will perform the experiment, write your lab report and include the answers to the loanitional 4 questions within the textual matter for full credit on this experiment.
Materials:
* 3% hydrogen peroxide
* a household blender
* potatoes (3 medium size potatoes, that ar peeled and quartered)
* lean water
* 8 test tubes
* 8 transfer pipets
* sugar packets
* salt
* a small saucepan
* a refrigerator with a freezer
* stove
* 4 blow over plastic cups
* tongs (to remove test tubes from boiling water)
* 4 coffee cups
* cheesecloth to filter the potato extract
* a wax pencil or Sharpie marker
* a ruler
* scale or balance
* 250 ml graduated cylinder
* oven mitt or tongs
Preparation Step 1: Research (online) the reaction between catalase and hydrogen peroxide and be familiar with the reactants, products, and enzyme. You will include the overall reaction in your lab report for this experiment.
We will measure enzyme activity by mensuration the height of the bubbles produced.
Questions
1. Given your research, why are we measuring the height of the bubbles produced to indicate how much enzyme activity has occurred?
2. What product are we specifically observing being produced?
Preparation Step 2:
1. Prepare the potato extract (catalase) using the following proficiency:
* peel 3 medium size potatoes and cut them into quarters
* add the potatoes to the blender with 250 mls of tap water to cover the potatoes and blend on high until the potatoes are pureed (blended to a thick liquid or paste)
* place a cheesecloth over a container and use the cheesecloth to come up the liquid portion (only) of your potato puree. You can...If you want to get a full essay, order it on our website: Ordercustompaper.com
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