Determining The Properties Of An Enzyme

DETERMINING THE PROPERTIES OF AN ENZYME














I.Abstract
Enzymes are responsible for the speed at which chemical reactions they are involved in take place. This experiment determines the do that concentration, temperature, pH, and boiling meet on an enzymes ability to perform its work. It is hypothesized that none of these variables will have any effect on the action mechanism of enzymes and these hypotheses are tested utilize dye-coupled reactions to determine the respect at which peroxidase converts H2O2 into water (H2O) and oxygen (O2). Each hypothesis is later rejected as data suggests that concentration, temperature, pH, and boiling all have an effect on enzyme activity.

II.Introduction

function 1: The Effects of engrossment on Enzyme Activity
In this experiment, the effectuate of concentration on enzyme activity were determined by using a spectrophotometer to solemnize absorbance changes victorious place in samples containing a pH 5 buffer, guaiacol, henry peroxide, and different volumes of murphy extract.

Hypotheses
Ho: The amount of enzyme added does not bend the rate of reaction.
Ha: The amount of enzyme added does lure the rate of reaction.





cleave 2: The Effects of Temperature on Enzyme Activity
In this experiment, the effects of temperature on peroxidase activity were determined by using a spectrophotometer to observe absorbance changes taking place in temperature treated samples containing potato extract, guaiacol, hydrogen peroxide, and pH 5 buffer.

Hypotheses
Ho: The temperature of solution does not influence the activity of an enzyme.
Ha: The temperature of solution does influence the activity of an enzyme.

Part 3: The Effects of pH on Enzyme Activity
In this experiment, the effects of pH on peroxidase activity were determined by using a spectrophotometer to observe absorbance changes taking place in samples containing potato extract, guaiacol, hydrogen peroxide, and buffers of different...If you want to get a full essay, aver it on our website: Ordercustompaper.com



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